H3284

Sigma

 

Anti-Histone Deacetylase 1 (HDAC1) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

MDL number:MFCD01633420

Description

Immunogensynthetic peptide corresponding to amino acids 466-482 of human and mouse HDAC1.
Physical formSolution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody formIgG fraction of antiserum
clone(polyclonal)
formbuffered aqueous solution
mol wtantigen mol wt 65 kDa
species reactivitymouse, human
application(s)immunoblotting: 1:20,000 using nuclear extract from HeLa human epithelioid carcinoma cells
 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human lymph node sections
 immunoprecipitation: 5-10 μL using whole lysate of NIH3T3 cells
 microarray: suitable
Gene Informationhuman ... HDAC1(3065)
 mouse ... Hdac1(433759)
shipped indry ice
storage temp.−20°C

Safety

WGK GermanyNWG

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References

referenceCaretti, G., et al., Dynamic recruitment of NF-Y and histone acetyltransferases on cell-cycle promoters. J. Biol. Chem. 278, 30435-30440, (2003) Abstract
 Gurtner, A., et al., Direct p53 Transcriptional Repression: in vivo Analysis of CCAAT-Containing G2/M Promoters Mol. Cell. Biol. 25, 3737-3751, (2005) Abstract
 Sacchi, A., et al., NF-Y Dependent Epigenetic Modifications Discriminate between Proliferating and Postmitotic Tissue PLoS ONE 3, e2047, (2008) Abstract